human triple negative breast cancer cells  (ATCC)

Journal: BMC Pharmacology & Toxicology

Article Title: Integrated in silico and in vitro evaluation of Genistein and Apigenin as dual inhibitors of PARP1 and ESR1 in breast cancer

doi: 10.1186/s40360-025-01082-z

Figure Lengend Snippet: Dose-dependent cytotoxic effects of GNT and Apigenin on HCC1937 cells evaluated by the MTT assay. ( A ) Dose–response curves showing percentage cell viability plotted against the logarithm of compound concentration (µM). ( B ) Bar graph representation of cell viability at increasing concentrations (10–400 µM) compared to control. Data are presented as mean ± SD ( n = 6)

Article Snippet: HCC1937 human triple-negative breast cancer (TNBC) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA; Cat. No. CRL-2336).

Techniques: MTT Assay, Concentration Assay, Control

Journal: Cell Death & Disease

Article Title: Dexamethasone drives macrophage repolarization linked to increased triple-negative breast cancer aggressiveness

doi: 10.1038/s41419-025-08363-9

Figure Lengend Snippet: Immunoassay analysis of A IL-1β, B IL-6, C IL-18, D IL-23, E IL-27, F GM-CSF, G TNF-α, H TNF-β, I FLT-1, J IL-10, K VEGF-C, L bFGF, and M SAA1, upon 8 days of 100 nM DEX (M1 DEX) or vehicle (EtOH) treatment during TPH-differentiation to M1 polarized macrophages, or vehicle (EtOH) treatment during differentiation to M2 polarized macrophages ( n = 5). N MDA-MB-231 cell Matrigel invasion in response to conditioned medium from unpolarized macrophages (M), M1 polarized macrophages (M1), DEX-treated polarized macrophages (M1 DEX), M2 polarized macrophages (M2), and to 10% FBS-containing medium (positive control) ( n = 3). O Effect on MDA-MB-231 proliferation upon 2-day incubation with conditioned medium from M1, M1 DEX, M1 DEX medium with 10 µM GR-inhibitor relacorilant (REL), and medium from M2 macrophages (n = 3). P Matrigel invasion and Q proliferation of MDA-MB-468 cells in response to the same conditioned medium as in N and O ( n = 4). Bars represent mean ± SD in A – M , or ±SEM in ( N , O , P , Q ). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Statistical significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons correction, or an unpaired Student’s t -test in ( J ).

Article Snippet: The human monocytic cell line THP-1, human triple-negative breast cancer (TNBC) MDA-MB-231 cells, human TNBC MDA-MB-468 cells, and the mouse TNBC luciferase-expressing 4T1-Luc2 cells were obtained from ATCC (#TIB-202, #HTB-26, #HTB-132, #CRL-2539-LUC2; respectively) and propagated in ATCC-modified RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (both from Gibco; #A1049101, #10500064; respectively).

Techniques: Positive Control, Incubation